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蘭花 蜘蛛百合 金英樹
* :::* 你現在的位置:首頁 > Faculty > Yen-Chung Lee, Associate Professor
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友善列印


Yen-Chung Lee                                 

Assistant Professor

TEL: 05-2717773
FAX: 05-2717755
E-mail:leeyc@mail.ncyu.edu.tw

Education

1995, B.S. Department of Chemstry, Tamkang University,  Taipei, Taiwan

1997, M.S. Department of Agricultural Chemistry, National Taiwan University,Taipei, Taiwan

2007, Ph.D. Institute of Molecular Biology, National Chung Hsing University, Taichung, Taiwan

Professional Experience

1999.08~2001.07, Research assistant, Institute of Molecular Biology, National Chung Hsing University, Taichung, Taiwan

2007.02~2008.07, Post doctoral fellow, Institute of Biochemistry, National Chung Hsing University, Taichung, Taiwan

2008.09~2009.01, Post doctoral fellow, Institute of Biotechnology, National Cheng Kung University, Tainan, Taiwan

2009.02~present, Assistant Professor, Department and Institute of Bioagricultural Technology, National Chiayi University, Chiayi, Taiwan
 
Research Interests

1. Technological platforms: Gene over-expression in Escherichia coli and Pichia pastoris, biotechnology, enzymology, and protein engineering.

2. Protein X-Ray crystallography and protein structure biology.

3. Development of the bioconversion system for the manufacture of pharmaceutical drugs.

Publications:

1. Lee, Yen-Chung*; Lin, Den-Tai; Chen, Hsiang-Ling; Lo, Huei-Fen; Hu, Hui-Yu; Ong, Ping-Lin; Lin, Long-Liu*. (2012) Design and characterization of glycine substitution mutations within the putative NAD+-binding site of Bacillus licheniformis aldehyde dehydrogenase. Protein engineering design and Slection. (In press) (SCI)
 
2. Hui-Fen Liao, Chao-Hung Kao, Wei-De Lin, Wen-Hwei Hsu, Y. C. Lee*. (2012). N-Acetyl-D-glucosamine 2-epimerase from Anabaena sp. CH1 contains a novel ATP binding site required for catalytic activity. Process Biochemistry. (In press) (SCI).
 
3. Y. C. Lee, Den_Tai Lin, Ping_Ling Ong,Hsiang_Ling Chen, Huei_Fen Lo, and Long_Liu Lin*. (2011). Contribution of Conserved Glu255 and Cys289 Residues to Catalytic Activity of Recombinant Aldehyde Dehydrogenase from Bacillus licheniformis. Biochemistry (Moscow) 76, No.11, 1233-1241 (SCI).
 
4. K H. Chin, Y. C. Lee, Z. L Tu1, C. H. Chen, Yi-Hsiung Tseng, Jinn-Moon Yang, Robert P. Ryan, Yvonne McCarthy, J. Maxwell Dow, Andrew H.-J. Wang and Shan-Ho Chou*. (2010). The cAMP Receptor-Like Protein CLP Is a Novel c-di-GMP Receptor Linking Cell–Cell Signaling to Virulence Gene Expression in Xanthomonas campestris. Journal of Molecular Biology  396, 646-62 (SCI).
 
5. Y. C. Lee, H. R. Chien, and W. H. Hsu*. (2007). Production of N-acetyl-D-neuraminic acid by recombinant whole cells expressing Anabaena sp. CH1 N-acetyl-D-glucosamine 2-epimerase and Escherichia coli N-acetyl-D-neuraminic acid lyase. Journal of Biotechnology 129, 453-460 (SCI).
 
6. Y. C. Lee, H. M. Wu, Y. N. Chang, W. C. Wang*, and W. H. Hsu*. (2007). The central cavity from the (Alpha/Alpha)6 barrel structure of Anabaena sp. CH1 N-acetyl-D-Glucosamine 2-epimerase contains two key histidines for reversible conversion. Journal of Molecular Biology 367, 895-908 (SCI).
 
Patent

1. Y. C. Lee, and W. H. Hsu. 2007. Method and application of N-acetyl-D-neuraminic acid production. R.O.C. patent, I 323175. 
 

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